Composite

Part:BBa_K1688013

Designed by: Mikael Andersson Schönn   Group: iGEM15_Uppsala   (2015-09-15)
Revision as of 18:05, 18 September 2015 by Kiira (Talk | contribs)


Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23110 promoter)

Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida + BBa_J23110 promotor and B0034 RBS. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4182
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
    Illegal NotI site found at 8225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5275
    Illegal BamHI site found at 3260
    Illegal BamHI site found at 5962
    Illegal XhoI site found at 1371
    Illegal XhoI site found at 3759
    Illegal XhoI site found at 3911
    Illegal XhoI site found at 9413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
    Illegal NgoMIV site found at 1864
    Illegal NgoMIV site found at 3639
    Illegal NgoMIV site found at 5513
    Illegal NgoMIV site found at 6125
    Illegal NgoMIV site found at 9438
    Illegal AgeI site found at 702
    Illegal AgeI site found at 3633
    Illegal AgeI site found at 6341
    Illegal AgeI site found at 8336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2981
    Illegal BsaI site found at 3654
    Illegal BsaI site found at 4245
    Illegal BsaI site found at 5411
    Illegal BsaI.rc site found at 2815
    Illegal BsaI.rc site found at 5565
    Illegal BsaI.rc site found at 7850
    Illegal SapI.rc site found at 3345
    Illegal SapI.rc site found at 3891
    Illegal SapI.rc site found at 7742


[edit]
Categories
Parameters
n/aNah7 - Upper naphthalene breakdown pathway (inc RBS, J23110 promoter)