Part:BBa_K1675000

NhaA; sodion-proton antiporter; membrane protein

The gene, NhaA, is the archetypal sodium/proton anti-porter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Lithium-ion concentration. NhaA catalyzes the exchange of 2 protons per soduim ion. The anti-porter active at alkaline pH, whose activity is strongly down-regulated below pH 6.5. Therefore, NhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 261
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 458
  Illegal BamHI site found at 995
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 321
  
• 1000
  COMPATIBLE WITH RFC[1000]