Coding

Part:BBa_K1602011

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-14)
Revision as of 17:41, 18 September 2015 by Lenuchka (Talk | contribs)

2-Dehydro-3-deoxy-D-pentonate aldolases - yagE

yagE is coding for a 2-dehydro-3-deoxy-D-pentonate aldolases from E. coli. It catalyzes the reaction of 2-dehydro-3-deoxy-D-pentonate to glycoaldehyde and pyruvate.
https://static.igem.org/mediawiki/parts/6/62/BBa_K1602011_image_1.png

Characteristics


Molecular Weight 32530.40
Residues 302
Charge -0.5
Isoelectric Point 6.4046
A280 Molar Extinction Coefficients 21430 (reduced) 21555 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175
[edit]
Categories
Parameters
None