Generator

Part:BBa_K1713001:Design

Designed by: Xudong Luo   Group: iGEM15_HZAU-China   (2015-09-14)
Revision as of 17:37, 18 September 2015 by Luoxudong (Talk | contribs) (→‎References)

Plac/ara-1+strong RBS+LacI(+LVA)+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1275
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A

Source

The promoter was standardized based on a earliest paper of 1997 while other parts were all from iGEM distribution.

References

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210. </br> Stricker J, Cookson S, Bennett M R, et al. A fast, robust and tunable synthetic gene oscillator[J]. Nature, 2008, 456(7221): 516-519.