Generator

Part:BBa_K1713004

Designed by: Xudong Luo   Group: iGEM15_HZAU-China   (2015-09-14)
Revision as of 17:30, 18 September 2015 by Luoxudong (Talk | contribs)

Plac/ara-1+strong RBS+mRFP+double terminator

The hybrid promoter ( Plac/ara-1 ) can be activated by the AraC protein in the presence of arabinose and so that mRFP produced. And then the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None