Regulatory
GAL1

Part:BBa_J63006

Designed by: Caroline Ajo-Franklin   Group: Silver Lab   (2006-10-11)
Revision as of 17:14, 18 September 2015 by SoanAnna (Talk | contribs)

yeast GAL1 promoter

GAL1 promoter from S. cerevisiae Contains Kozak sequence, part BBa_J63003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Promoter characterization

Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the [Part:BBa_J63006:Experience|Experience page] for details.

Promoter Strength

JHU Igem 2011 is working on further characterization of this part through using GFP expression within the next month.


Promoter dose =

Gal1 prevents growth in media containing galactose.

SCUT iGEM 2014 have designed a experiment to quantify Gal1 dose titer.


Figure 1.This year we have .
[edit]
Categories
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
Parameters
negative_regulators
positive_regulators1