Regulatory
Part:BBa_K1723001:Experience
Designed by: Emilie Cuillery Group: iGEM15_EPF_Lausanne (2015-09-08)
Revision as of 17:14, 18 September 2015 by E.Cuillery (Talk | contribs)
Applications of BBa_K1723001
This picture represent the system we built using this promoter : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.
Effects of of dCas9-ω (BBa_K1723000) on BBa_K1723001 promoter
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:
[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting
In comparison with:
[B] apo-dCas9-ω (=no sgRNA)
[Af] no dCas9-ω, no sgRNA
This chart show that BBa_K1723000 can act as a transcription regulator on promoter BBa_K1723001 by either activating or inhibiting it. We also see that when provided both gRNA Z4, activating, and gRNA Z35, inhibiting, BBa_K1723001's inhibition is stronger, then allowing the creation of our transistor-like elemens. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne/Results
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UNIQ598bb3a3d75fcff9-partinfo-00000000-QINU UNIQ598bb3a3d75fcff9-partinfo-00000001-QINU