Part:BBa_K1593666

SARS Coronavirus Envelope Protein

This part is SARS Coronavirus Envelope Protein, abbreviated as SCVE. The protein will improve the bactial permeability, which is one of the strategy of permeability improvement originally inplemented in USTC 2015.

Description

SCVE, the abbreviation of SARS Caronavirus Envolope protein,is a kind of effective viroporin(viroporins are similar to porins in that they oligomerize within the membrane to form pores and this oligomerization often occurs between hydrophobic, transmembrane and alpha-helical domains of the proteins) within 76 residues that is found normally pentamerized in bacteria. SCVE is permeable for many small molecules, which is originately designed for apoptosis of cells triggered by virus. Implementation of SCVE in ROSE may potentially improve the permeability of small molecules including antibiotics.

76 residues of SCVE.SCVE is able to form TM homo-oligomers

Pentameric protein

The evidence to show that SCVE is found normally pentamerized in bacteria

The pentameric form of SCVE

Viroposin as Iron Channel

SCVE protein is permeable for small molecule, also for ion(as iron channel), especially for cation ion.

The micro electric potential change during the ion transport process

Experimental Result

We had already done the polymerase chain reaction (PCR) of SCVE successfully.

Reference

1. Model of a putative pore: the pentameric alpha-helical bundle conronavirus E protein in lipid bilayers Biophysics(2006) DX Liu.
2. SARS coronavirus E protein forms cation-selective ion channel Virology(2004) Gary Ewart. Sequence and Features BBa_K1593666 SequenceAndFeatures