Translational_Unit

Part:BBa_K1688002

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)
Revision as of 16:54, 18 September 2015 by Kiira (Talk | contribs)

RhlA Gene

The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene codes for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomona aeruginosa. The biobrick BBa_K1688002 is an assembly of RBS with BBa_K1331001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
    Illegal BamHI site found at 647
    Illegal XhoI site found at 823
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312
    Illegal BsaI.rc site found at 496


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Categories
Parameters
None