Coding

Part:BBa_K1723000:Experience

Designed by: Gregoire Thouvenin   Group: iGEM15_EPF_Lausanne   (2015-08-10)
Revision as of 16:54, 18 September 2015 by E.Cuillery (Talk | contribs) (→‎Applications of BBa_K1723000)


Applications of BBa_K1723000




EPF_Lausanne_transistor.png

This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.




Effects of BBa_K1723000 on BBa_K1723001 promoter

EPF_Lausanne_RESULTS_EXPERIENCE.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:

[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting

In comparison with:

[B] apo-dCas9-ω (=no sgRNA)
[Af] no dCas9-ω, no sgRNA




Effects of BBa_K1723000 on BBa_K1723005 promoter

EPF_Lausanne_RESULTS_EXPERIENCE2.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:

[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting

In comparison with:

[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] no dCas9-ω, no sgRNA

Those charts show that BBa_K1723000 can act as a transcription regulator on both promoters BBa_K1723001 and BBa_K1723005 by either activating or inhibiting them.





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