Translational_Unit

Part:BBa_K515001

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 16:38, 18 September 2015 by Qiuxinyuan12 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

IaaH - Indoleacetamide hydrolyase

Indoleacetimide hydrolase catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. The enzyme originates from the plant pathogen Pseudomonas savastanoi which produces the auxin, indole-3-acetic acid via a two step pathway.

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

For the full characterisation of the device, please refer to the BBa_K515100 page.



Contribution: NUDT_CHINA 2015

Author: Xinyuan Qiu

Summary: We built a new part based on this part to extend its function.

We designed a new part by removing the RBS from this part to extend its usage.

A new Bio-brick was designed based on this part

Primers used to build the new part:

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’

R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’


For further information,

See BBa_K1789001

This part was sent to the registry.

[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli DH5α
device_typepathway
input_sIndole acetamide
originP. savastanoi
outputindole-3-acetic acid
resistancechloramphenicol