Composite

Part:BBa_K1675010

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-15)
Revision as of 16:37, 18 September 2015 by JIAQIXU (Talk | contribs)

FimE recombinase device

FimE is different from other kinds of recombinase. Its recognized sites on the upstream (IRL) and downstream (IRR) are not same. It means that after the recombination mediated by FimE happens, the sign sites would be exchanged, so that the new sign sites couldn’t be recognized by FimE again. So the recombination mediated by FimE is irreversible. Granted that the bacteria produce the strong alkali in the primary, the environment would turn alkaline quickly. So P-atp2 can correspond the change of environment and start the expression of FimE. FimE recognizes IRL and IRR, then catalyze the inversion of J23119. The weak acid would be generated at that time. For the environment, due to the production of the weak acid, it would turn neutral gradually.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None