Part:BBa_K515000

IaaM - tryptophan-2-mono-oxygenase IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, Pseudomonas savastanoi

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 450
  Illegal BamHI site found at 1395
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 157
  
• 1000
  COMPATIBLE WITH RFC[1000]

This BioBrick has been sequence verified.

For the full characterisation of the device, please refer to the BBa_K515100 page.

References

[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448

Contribution: NUDT_CHINA 2015

Author: Xinyuan Qiu

Summary: We built a new part based on this part to extend its function.

We designed a new part by removing the RBS from this part to extend its usage.

A new Bio-brick was designed based on this part

Primers used to build the new part:

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’

R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’

For further information,

See BBa_K1789000

This part was sent to the registry.