Composite

Part:BBa_K1714001:Experience

Designed by: Masaya Mitsumoto   Group: iGEM15_HokkaidoU_Japan   (2015-09-10)
Revision as of 16:24, 18 September 2015 by NOnoda (Talk | contribs) (→‎Applications of BBa_K1714001)


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Applications of BBa_K1714001

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.

Activity test for ABF-2 and thanatin

→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

  1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
  2. Adding 2 µL of IPTG into one and DW into the other.
  3. Measuring OD600 of these samples once in an hour.

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