Part:BBa_J31007

tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_J31007 is the coding region for tetracyline resistance [TetA(C) inner membrane associated protein] in the forward orientation. This does not code for the repressor of the tetracycline promoter. This part requires a promoter and RBS for proper expression.

Usage and Biology

Cells expressing BBa_J31007 are able to grow on media containing tetracycline, however overexpression is detrimental to cell growth.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 144
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 290
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 316
  Illegal NgoMIV site found at 684
  Illegal NgoMIV site found at 844
  
• 1000
  COMPATIBLE WITH RFC[1000]