Part:BBa_K1725301

Member of the RBS library - derived from BBa_B0032

Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR.

Identifier	Sequence	Estimated strength (au)
Degenerate sequence	TCACACANRARRG	-
BBa_K1725301	TCACACAGGAGGG	15730.86
BBa_K1725302	TCACACAGGAGAG	12493.41
BBa_K1725303	TCACACAGGAAGG	8716.05
BBa_K1725304	TCACACAAGAGGG	7247.43
BBa_K1725305	TCACACAAGAAGG	5532.39
BBa_K1725306	TCACACACGAGGG	3880.58
BBa_K1725307	TCACACATGAGGG	3880.58
BBa_K1725308	TCACACAAAAAGG	2574.21
BBa_K1725309	TCACACAAAAGGG	2574.21
BBa_K1725310	TCACACACAAAGG	2574.21
BBa_K1725311	TCACACACAAGGG	2574.21
BBa_K1725312	TCACACAGAAAGG	2574.21
BBa_K1725313	TCACACATAAAGG	2574.21
BBa_K1725314	TCACACATAAGGG	2574.21
BBa_K1725315	TCACACATGAAGG	2574.21
BBa_K1725316	TCACACACGAAGG	2352.63
BBa_K1725317	TCACACAGGAAAG	2161.77
BBa_K1725318	TCACACAAGAGAG	1969.19
BBa_K1725319	TCACACATGAGAG	1506.8
BBa_K1725320	TCACACACGAGAG	1500.04
BBa_K1725321	TCACACAGAAGGG	1203.18
BBa_K1725322	TCACACAAAAGAG	875.94
BBa_K1725323	TCACACACAAGAG	875.94
BBa_K1725324	TCACACAGAAGAG	875.94
BBa_K1725325	TCACACATAAGAG	875.94
BBa_K1725326	TCACACAAGAAAG	355.35
BBa_K1725327	TCACACAAAAAAG	271.26
BBa_K1725328	TCACACACAAAAG	271.26
BBa_K1725329	TCACACAGAAAAG	271.26
BBa_K1725330	TCACACATAAAAG	271.26
BBa_K1725331	TCACACATGAAAG	271.26
BBa_K1725332	TCACACACGAAAG	158.07

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]