Coding

Part:BBa_K1797011

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)
Revision as of 16:01, 18 September 2015 by Morning ZHOU (Talk | contribs)

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RecA

RecA is an essential protein for DNA repair and maintenance. Researchers (Stahl F W, Stahl M M, Malone R E. Red-mediated recombination of phage lambda in a recA− recB− host[J]. Molecular and General Genetics MGG, 1978, 159(2): 207-211.) have found that red recombination system works better in recA+ strains. To maximize the efficiency of red recombination system, we need to introduce recA exogenously in plasmid form though recA is intrinsically existent in E.coli. To have a clearer view of red recombination system, you can refer to BBa_K1797014 and BBa_K1797013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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