![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1610105
Designed by: Leon Yim Group: iGEM15_TAS_Taipei (2015-09-06)
Revision as of 15:41, 18 September 2015 by Leony16111710 (Talk | contribs)
pTS + RBS + yebF + ACT3m + Term
This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a motor protein sequence yebF (Bba_K1610300), a coding sequence (Bba_K1610201), and a terminator (Bba_B0015). This part is used to produce and secrete a modified human antichymotrypsin, a human extracellular protein that has been given the ability to bind and inhibit Granzyme B. This circuit is temperature regulated, where environments at or above 37C will activate the promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1082
Illegal BglII site found at 1913
Illegal XhoI site found at 2288 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2214
[edit]
Categories
Parameters
//awards/composite_part/nominee
None |