Part:BBa_K1587001

acyl-CoA thioesterase 2

Figure 1 Butyrate formation catalyzed by tesB

The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to Escherichia coli, its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

Used with other genes to produce butyrate in Escherichia coli (Figure 2)

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 542
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 145
  Illegal SapI.rc site found at 157