Translational_Unit

Part:BBa_K1679013

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-07)
Revision as of 15:18, 18 September 2015 by QinQikai (Talk | contribs)

RNA thermometer (ROSE)+mRFP

This is a device containing a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When the temperature rises above 42°C,It can produce RFP.However,according to our experimental,It can't appear red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None