Translational_Unit

Part:BBa_K1587000

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-13)
Revision as of 14:54, 18 September 2015 by MathildeBeraud (Talk | contribs)

ho1 with RBS

Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022). ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB) (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

TLSE ho1.jpg
Figure 1: pcyA catalysed formation of PCB from biliverdin (BV) IXα. (Ken et al. 2009)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
chassisE. coli
proteinHeme oxygenase 1
rbsRBS.1 (strong) -- modified from R. Weiss