Part:BBa_K1789013

SCAF1+Ter

This is a combination of scaffold and termination.

Usage and Biology

Usage: This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.

Biology: Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 145
  Illegal AgeI site found at 175
  Illegal AgeI site found at 205
  Illegal AgeI site found at 235
  Illegal AgeI site found at 265
  Illegal AgeI site found at 295
  Illegal AgeI site found at 325
  
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

Sequencing

This part is sequenced as correct after construction.

Enzyme digestion test

A double-enzyme digestion test was implemented to verify this part.

The length of the digested DNA fits the design.

Functional Parameters

CHIp

Enzyme digestion test /