Part:BBa_K608351:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_K608351

We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the insert was correct-, and preliminary OD-measurement testing of the part in conjuction with (BBa_K608352), as part of our temperature sensitive phage lysis cassette.

We later verified the part sequence. Please refer to our Project Results Page (http://2011.igem.org/Team:Freiburg/Results#Lysis_cassette) for more Info.

The OD values of the measurement are shown in the chart (please do bear in mind that this was a measurement done under deadline-stress, without the possibility to plan and perform something more concrete before the Wiki-Freeze)

LB with Cm (Chloramphenicol) was used as a blank before every (hourly) measurement

Nr1: Insert is only this part ((BBa_K608351))
Nr2: Insert is only (BBa_K608352)
Nr3: Both parts were correctly assembled (1:(BBa_K608351) + 2:(BBa_K608352))

Therefore (BBa_K608351) seems to be functioning, though not tightly. More tests are planned if time allows (ie wiki-unfreeze ;) ) One sure thing is that the correct testing would be done with GFP or RFP instead of (BBa_K608352), and also at different temperatures (probably a 25°C to 42°C gradient).

User Reviews

UNIQ2fd3343a3f30894b-partinfo-00000000-QINU UNIQ2fd3343a3f30894b-partinfo-00000001-QINU

••••• leony16111710

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