Part:BBa_K1583102

pRha + CsgA + Hydroxyapatite-affinity tag

CsgA with Hydroxyapatite-tag attachted to the C-terminus under control of L-rhamnose-inducible promoter ((BBa_K914003). CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli. This protein is transported as an unfolded protein out of the cell. Outside the cell CsgA proteins self-assemble into nanowires after nucleation on the membrane protein CsgB. CsgC prevents CsgA proteins from self-assembling inside the cell and the transport is ensured by the proteins CsgEFG. This part generates a peptide tag which shows high adhesive properties towards hydroxyapatite, a main component of e.g. teeth. (Roy et al. 2008) Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Roy, M.D. et al., 2008. Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display. Advanced Materials, 20(10), pp.1830–1836.