Composite

Part:BBa_K1797009

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)
Revision as of 14:00, 18 September 2015 by Morning ZHOU (Talk | contribs)

Red light sensing system with mRFP reporter

This part consists of a red light sensing system(BBa_K1797001) and an RFP reporter system. When bacterial colonies are exposed to red light, the OmpR promoter is not activated, preventing the expression of RFP. When colonies are kept in dark, the corresponding transcription factor of mpR promoter is activated and RFP is expressed, making the colonies red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3871
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3424
    Illegal AgeI site found at 4711
    Illegal AgeI site found at 4823
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None