Coding

Part:BBa_K1603003:Design

Designed by: John Hellgren   Group: iGEM15_Chalmers-Gothenburg   (2015-09-18)
Revision as of 13:41, 18 September 2015 by Jannie (Talk | contribs) (→‎Design Notes)


RecA - optimized for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1032


Design Notes

Synthetic gene ordered from IDT.

Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Primers for amplification with BioBrick Prefix in the FW primer and Suffix in the RV primer:

FW:GCTTCTAGATGTCTCATAGACATCACCATCAC

RV:AGCCTGCAGCGGCCGCTACTAGTATTAAGCTTCTGCGGCTTCA

Source

Sequence adapted from Deinococcus Radiodurans. Ordered as synthetic gene.

References