Regulatory

Part:BBa_K1603002:Design

Designed by: John Hellgren   Group: iGEM15_Chalmers-Gothenburg   (2015-09-18)
Revision as of 13:29, 18 September 2015 by Jannie (Talk | contribs) (→‎Design Notes)


Promoter pTPI1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer: FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG

RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT


The primers were designed to remove the EcoRI site from the insert when constructing the biobrick to reduce the amount of base pairs in the primers. The primers also add 3 extra protective basepairs to the PstI site in the insert.


The construction of the final biobrick was initiated by cutting the vector (BBa_J04450) with XbaI, PstI, KnpI and FastAP and the insert with XbaI and PstI.

Source

Genomic sequence from Saccharomyces cerevisiae CEN.PK2

References