Composite

Part:BBa_K1799022

Designed by: Will Shindel, Michael Flanagan   Group: iGEM15_Genspace   (2015-09-17)
Revision as of 13:28, 18 September 2015 by EllenJ (Talk | contribs)

IPTG-Repressible RFP Generator

Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system. The device consists of the LsrR ORF under the control of the Lac promoter and a medium-strength RBS, followed by a terminator, followed by an RFP ORF under the control of pLsrA and a medium-strength RBS.

When cultured in a LacI- strain or in the presence of IPTG, LsrR is produced, repressing pLsrA and preventing expression of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1902
    Illegal AgeI site found at 2014
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None