Generator

Part:BBa_K1859024

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)
Revision as of 13:17, 18 September 2015 by Mori (Talk | contribs)

mRNA circularize efficiently generator (outside complementarity)

We designed this generator [K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004]. RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_1859026] was the third, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).

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