Part:BBa_K1859024
mRNA circularize efficiently generator (outside complementarity)
We designed this generator [K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004]. RNA sequences of these linkers bind complementary with each other in circular mRNA.
We found that circular efficiency of [BBa_1859026] was the third, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).
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