Generator

Part:BBa_K1713015:Design

Designed by: Keji Yan   Group: iGEM15_HZAU-China   (2015-09-14)
Revision as of 13:16, 18 September 2015 by Luoxudong (Talk | contribs) (→‎Source)

luxpR+B0034+LuxI(-LVA)+B0032+AiiA(-LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 850
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag.

Source

All the basic parts come from iGEM distribution.

References