Composite

Part:BBa_K1689000

Designed by: WEI Weijia   Group: iGEM15_Peking   (2015-09-16)
Revision as of 13:03, 18 September 2015 by Moximonx (Talk | contribs)

sgRNA generator

including T7 promotor, LacZ a', crRNA (tracrRNA) and T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 425
    Illegal BsaI.rc site found at 21


[edit]
Categories
Parameters
None