Composite

Part:BBa_K1736300:Design

Designed by: Nick Coleman   Group: iGEM15_Sydney_Australia   (2015-09-17)
Revision as of 12:59, 18 September 2015 by SydneyUni Australia (Talk | contribs) (→‎Design Notes)


LacI-LacIQ1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PLacIQ1 promoter is placed upstream of RBS and LacI sequence. It is a strong consitutive promoter 1. The RBS is from wild type E.coli.

Source

Bacterial LacI and LacIQ1 strong constitutive promoter developed by Glascock et al.

References