Composite

Part:BBa_K1638009:Design

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-19)
Revision as of 12:52, 18 September 2015 by Jpettersen (Talk | contribs) (→‎Design Notes)


T18 domain of cyaA from Bordetella pertussis with GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal NgoMIV site found at 662
    Illegal AgeI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1384


Design Notes

This part was assemblied by the part containing the T18 domain (BBa_K1638005) and the linker-GFP BBa_K1638007. These parts respectively contains a BamHI res. site in the 3'-end and the 5'-end allowing assembly without creating a scarsite. Scarsite includes a stop-codon, which is not favorable for correct of assembly of fusion proteins.

Source

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References