Composite

Part:BBa_K1736000:Design

Designed by: Nick Coleman   Group: iGEM15_Sydney_Australia   (2015-09-17)
Revision as of 12:44, 18 September 2015 by SydneyUni Australia (Talk | contribs) (→‎Design Notes)


Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 109
    Illegal BamHI site found at 229
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BsFP protein was harmonised by the method outlined in Spencer et al. 1 Note that the part also contains an B.subtilis RBS.

1 Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.

Source

Bacillus Subtilits

References