Regulatory

Part:BBa_K1603002:Design

Designed by: John Hellgren   Group: iGEM15_Chalmers-Gothenburg   (2015-09-18)
Revision as of 12:38, 18 September 2015 by Jannie (Talk | contribs) (→‎Design Notes)


Promoter pTPI1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer: FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG

RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT

Source

Genomic sequence from Saccharomyces cerevisiae CEN.PK2

References