Composite

Part:BBa_K1638009

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-19)
Revision as of 12:36, 18 September 2015 by Jpettersen (Talk | contribs)

T18 domain of cyaA from Bordetella pertussis with GFP reporter T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This part allows detection of expression of the gene encoding the T18-GFP fusion protein. Additionally, the presence of green fluorescence will verify correct folding of the protein fused to the T18-domain through the flexible aa linker. Correct folding of our T18-domain is obviously improtant for the bacterial two-hybrid system to work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal NgoMIV site found at 662
    Illegal AgeI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1384


[edit]
Categories
Parameters
None