Coding

Part:BBa_K1593209

Designed by: Juntao Yu   Group: iGEM15_USTC   (2015-08-17)
Revision as of 12:10, 18 September 2015 by Yukae (Talk | contribs) (→‎electrophorogram)

Porin: OprF from Pseudomonas aeruginosa

OprF is a kind of porin on the outer membrane of Pseudomonus aeruginosa. OprF represents one of the largest pore sizes on bacterial outer membranes, allowing diffusion of polysaccharides in a range of 2000 to 3000 Da. The overexpression of OprF in E. coli will drastically improve the antibiotic absorption capability, like antibiotic condensation occured in E. coli.


electrophorogram

File:Https://static.igem.org/mediawiki/2015/6/66/USTC-OprF.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 582
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None