Part:BBa_K1686033:Experience

Applications of BBa_K1686033

To verify that the OsmY promoter is only active in stationary phase, we realized Curdlan quantitative analyses every hour of a culture in LB medium. We could therefore show a parallel between growth and Curdlan production. The culture temperature was changed when stationary phase was obtained.

→ As we can see, Curdlan appears after the switch at 27°C. So, OsmY promoter is active in stationary phase only.

Fig.1: OsmY promoter characterization : The production of Curdlan was made in LB medium at 37°C during exponential phase and after a temperature switch at 27°C at the beginning of the stationary phase (to slow down cell metabolism). The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans.

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