Regulatory

Part:BBa_K1603001:Design

Designed by: John Hellgren   Group: iGEM15_Chalmers-Gothenburg   (2015-09-18)
Revision as of 11:57, 18 September 2015 by Registry (Talk | contribs)

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pTEF1-pSUC2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167


Design Notes

Added 20 overlapping base pairs between the two gene fragments through PCR with primers with overhangs. This allowed the two promoters to be combined through Gibson assembly. Added Prefix and Suffix through PCR with primers with overhangs.


Source

Genomic sequence from Saccharomyces cerevisiae CEN.PK2

References