Composite
Part:BBa_K1642009:Design
Designed by: Can Yin Group: iGEM15_SJTU-BioX-Shanghai (2015-09-05)
Expression of GFP under the control of Pdark
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 611
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2995
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1294
Design Notes
Pdark
Pdark-GFP
Source
ClonExpress technique of Vazyme Biotech Co.,Ltd from composite Pdark-HR(BBa_K1642011) and GFP gene sequence.