Composite

Part:BBa_K1638037

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-09-14)
Revision as of 10:40, 18 September 2015 by Jpettersen (Talk | contribs)

CsrA fused to T25 domain of CyaA with cAMP-induced RFP reporter

The carbon storage regulator CsrA fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between CsrA and another x protein fused to the T18 domain. If a library of peptide aptamers is fused to the T18-domain, one can screen for specific peptide aptamers against CsrA [1].

Click in on the following parts to dig deeper into the two-hybrid screening and learn how to construct your own experiment for detection of protein-protein interactions:
BBa_K1638033
BBa_K1638005

If you want to do a screening for peptide aptamers we recommend the following parts:
BBa_K1638018
BBa_K1638035

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.


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