Coding

Part:BBa_K1640021

Designed by: Meghan Cook   Group: iGEM15_Macquarie_Australia   (2015-09-18)
Revision as of 10:04, 18 September 2015 by Mikeee (Talk | contribs) (→‎Biology & Literature)

psbWK

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


Overview

This biobrick contains two genes from the photosystem II complex of Chlamydomonas reinhardtii - psbW and psbK, with RBS preceeding each gene, and a terminator at the 3' end.

This part was designed in conjunction with BBa_K1640008, and together they form operon 4 in our set of photosystem II operons:

PSII diagram

Biology & Literature

This part is composed of psbW and psbK genes. The psbW protein stabilizes dimeric Photosystem II. The psbK protein is also required for stability and assembly of Photosystem II.

Protein information

psbW

mass: 9.2kDa

sequence: MATTVRSEVAKKVAMLSTLPATLAAHPAFALVDERMNGDGTGRPFGVNDPVLGWVLLGVFGTMWAIWFIGQKDLGDFEDADDGLKL


psbK

mass: 5.0kDa

sequence: MTTLALVLAKLPEAYAPFAPIVDVLPVIPVFFILLAFVWQAAVSFR

References

[edit]
Categories
Parameters
None