Device
Part:BBa_K1789021
Designed by: Chushu Zhu Group: iGEM15_NUDT_CHINA (2015-09-14)
IAA prducer
This is a device which contains the sequence of the recombination of TALE1 and IAAM and the recombination of TALE2 and IAAH.
Usage and Biology
Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from Pseudomonas savastanoi. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.
In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are randomly distributed in cell without the scaffold. This is the negative control group of our project,aimed at making our theory more convincing.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2724
Illegal BamHI site found at 2311
Illegal BamHI site found at 3669
Illegal BamHI site found at 6072 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2431
Illegal NgoMIV site found at 7088 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1304
Illegal BsaI.rc site found at 5166
Illegal BsaI.rc site found at 5370
Experimental Validation
[edit]
Categories
Parameters
//awards/part_collection/2015
| chassis | E. coli BL21(DE3) |
| control | |
| device_type | pathway |
| input_s | tryptophan |
| origin | P. savastanoi |
| output | indole-3-acetic acid |
| resistance | chloramphenicol |