Part:BBa_K1789015
GFP_N1
This device is a negative control of GFP with scaf.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2311
Illegal BamHI site found at 4908 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1304
Illegal BsaI.rc site found at 3390
Illegal BsaI.rc site found at 3798
Illegal BsaI.rc site found at 4104
Illegal BsaI.rc site found at 5094
Functional check:
Methodology: In order to investigate whether the fluorescence
observed was due to the presence of the original GFP-N2 construction.
First of all, the recombinant colonies were transferred into
LB culture medium with chloramphenicol, and shook overnight at 37 ℃,
then took the medium for PCR templet. Meanwhile, we extracted plasmid DNA
using a miniprep kit and did a digest with Xbal1 and Pst1 to check
for the presence of the original insert and size of the unfolded vector, respectively.
Result and discussion: The DNA positive strand and the insert
is very clearly visible at just below 3kb and 5kb.
This proves the presence of GFP-N2 in culture.
| None |