Composite

Part:BBa_K1638031

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-08-18)
Revision as of 09:23, 18 September 2015 by Jpettersen (Talk | contribs)

Leucine zipper fused to T18 domain of cyaA with cAMP-induced RFP generator

This part consist of the leucine zipper region of the yeast GCN4 protein fused to the T18 domain as used in the bacterial two-hybrid system. This part allows test of functional complementation between the T18 and T25 domain. When T18-zip and T25-zip are co-transformed, the homodimerisation of the two leucine zipper will cause association of the T18 and T25 domain and catalyse formation of cAMP. By using the cAMP-induced reporter construct (mRFP generator controlled by the promoter PcstA), one can detect correct complementation by the appearance of red colonies [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1719
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1260
    Illegal NgoMIV site found at 1670
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
    Illegal AgeI site found at 1476
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.


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