Device

Part:BBa_K1789018:Design

Designed by: Dongyu Fan   Group: iGEM15_NUDT_CHINA   (2015-09-14)
Revision as of 09:04, 18 September 2015 by Fandongyu13 (Talk | contribs) (→‎Design Notes)

GFP_S1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2311
    Illegal BamHI site found at 4908
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5323
    Illegal AgeI site found at 5353
    Illegal AgeI site found at 5383
    Illegal AgeI site found at 5413
    Illegal AgeI site found at 5443
    Illegal AgeI site found at 5473
    Illegal AgeI site found at 5503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1304
    Illegal BsaI.rc site found at 3390
    Illegal BsaI.rc site found at 3798
    Illegal BsaI.rc site found at 4104
    Illegal BsaI.rc site found at 5094


Design Notes

This is the first experimental group of our project. Our theory is feasible if the functional parameter of this group is stronger than the group of negative control.

Split GFP is a technique that has been widely used in the research of protein-protein interaction. In our project, we demonstrated a prototype by fusing the Amino (or Carboxyl) Half of GFP with TALE1 (or TALE2/3).

By integrating the coding sequences of the TALE-fused proteins and the scaffold, three different plasmids can be constructed.


GFP SCAF1.jpg

Fig. 1 Split GFP fused with TALE1/TALE3 on SCAF1


This prototype is designed to test if our system can achieve our goal of compartmentation by examining if the green florescent intensity raised observably.

Fluoroskan Ascent FL by Thermo can be used to detect the fluorescence intensity.

Source

We obtained it through the contribution of our basic subparts.