Regulatory

Part:BBa_K1628001

Designed by: Xinhao Song   Group: iGEM15_Nankai   (2015-08-04)
Revision as of 08:56, 18 September 2015 by Songxinhao (Talk | contribs)

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Pbca

Promoter Pbca is the original promoter of pgsBCA operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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