Coding

Part:BBa_K1616013

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-18)
Revision as of 08:42, 18 September 2015 by Registry (Talk | contribs)

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ccdB reversed - RBS and double T7 terminator

ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison. The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, onlyDB3.1 is resistant.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 226


[edit]
Categories
//biosafety/kill_switch
Parameters
None