![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1616013
Designed by: Johanna Chesnel Group: iGEM15_IONIS_Paris (2015-09-18)
ccdB reversed - RBS and double T7 terminator
ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison. The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, onlyDB3.1 is resistant.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 226
[edit]
Categories
Parameters
//biosafety/kill_switch
None |