Part:BBa_K1632006

fim switch[default ON](Tokyo_Tech/R0010)

The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed the fim switch which has a J23119 promoter(BBa_K1632000) . Basically, the design of the fim switch(Tokyo_Tech) is similar to the fim switch(wild-type). The only difference is that we inserted restriction cut enzyme sites to fim switch(Tokyo_Tech). In detail, in fim switch(Tokyo_Tech) the sigma 70 promoter is exchanged to the J23119 promoter and there are two restriction enzyme cut sites inserted each in the front (SalI and BamHI) and in the back (BglII and MluI) of the promoter. Due to the insertion of the restriction enzyme cut sites, fim switch(Tokyo_Tech) has a promoter which can we exchanged with an arbitrary promoter. We actually changed J23119 promoter in the fim switch(Tokyo_Tech) to Lac promoter(BBa_B0010) as shown in the figure below.

Fig. 1. fim switch (Tokyo_Tech/B0010) design (Up:on state Down:off state)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 539
  Illegal BamHI site found at 333
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]