Composite

Part:BBa_K1813032

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-08-17)
Revision as of 08:29, 18 September 2015 by Rc (Talk | contribs)

LacIR, AgCPR and CYP2D6 Regulon

A construct used to achieve the bacterial expression of HUMCYPDB1 (BBa_K1813005) and the A. gambiae NADPH p450 reductase simultaneously (BBa_K1813010) under regulative authority of the LacI repressor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 1737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2327
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.

[edit]
Categories
Parameters
n/aLacIR AgCPR CYP2D6